FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8138603
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG13300.020.713778145
CGCCGTA45200.014.91667145
GCCGTAT45350.013.428557145
TGGTCGC81250.013.027374145
TCGCCGT65250.011.3330555145
TCTCGGT154600.010.973211145
GATCTCG196050.010.391227145
GTGGTCG92150.010.384987145
CCGTATC45900.010.266633145
AGTGTAG299950.010.248123145
GAGTGTA314500.010.004524145
GTGTAGA291900.09.98434145
CGTATCA41900.09.6895145
CGGTGGT122450.09.650642145
GGTCGCC91700.09.645348145
AGATCTC229050.09.558815145
GTCGCCG74150.09.386149145
CTCGGTG174700.09.337207145
TCGGTGG152700.09.258123145
AAAGAGT429650.09.229961145
Produced by FastQC (version 0.11.5)