FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6628152
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG11450.025.327234145
AGTGTAG155700.011.175215145
GTAGGGA316050.010.506134145
TGGTCGC38150.010.452048145
GTGTAGG351850.010.405599145
GTGTAGA152050.010.203768145
GCCGTAT21400.010.16344145
CGTGTAG361450.010.149286145
TAGGGAA295350.010.064219145
TCGTGTA383650.010.034427145
GTCGTGT407350.010.020143145
GTAGATC159350.09.963807145
GAGTGTA158600.09.645229145
GGGAAAG304300.09.625264145
CGTCGTG445000.09.547087145
AGCGTCG505900.09.472618145
GCGTCGT469550.09.433929145
GAGCGTC544400.09.415308145
AGAGTGT193300.09.41402145
TGTAGGG336400.09.374897145
Produced by FastQC (version 0.11.5)