FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7696449
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG19350.035.967896145
TGGTCGC53750.013.487961145
GTGGTCG63500.013.35786145
GTGTAGA249600.012.751013145
GCCGTAT27500.012.654159145
GAGTGTA276850.012.386294145
TAGATCT188700.011.948496145
AGAGCGG45050.011.7477145
AGTGTAG258750.011.6556835145
AGATCTC184500.011.395316145
TCTCGGT115050.011.342548145
CGCCGTA25650.011.305698145
GTCGCCG51350.011.29469145
GATCTCG148500.011.228613145
AGAGTGT324050.011.163831145
TAGGGAA527950.010.999285145
CCGTATC27800.010.952904145
TGTAGAT270100.010.951165145
GTGTAGG647700.010.756581145
CGGTGGT91350.010.634597145
Produced by FastQC (version 0.11.5)