FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4081389
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG8000.016.312267145
GATCTCG82350.014.70228145
TGGTCGC29950.014.523999145
TCTCGGT65800.013.662419145
GAGTGTA153950.013.091725145
CGCCGTA15950.012.727091145
TAGATCT107400.012.015656145
GTGTAGA135900.011.683057145
AGTGTAG139550.011.169672145
CGGTGGT50650.011.164698145
AGAGTGT177650.011.10032145
GTGGTCG36550.010.909557145
AGATCTC105950.010.674694145
GCCGTAT17100.010.599263145
TAGGGAA282650.010.080344145
GTAGGGA302100.010.055301145
GTAGATC148550.010.004906145
CCGTATC17450.09.971205145
CGTGTAG353850.09.936978145
TCGGTGG63550.09.925113145
Produced by FastQC (version 0.11.5)