FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7451490
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC52400.015.911396145
TCGCAAT55400.014.39543145
ATCGCAA72650.013.871421145
TATGCCG19150.013.629358145
AGATCGC100050.012.391411145
ATCTCGT39650.011.702497145
GATCGCA80650.011.596503145
CGTCTGA458450.011.180704145
CGTATGC25300.010.602858145
TCGTATG29150.010.446059145
ACACGTC569250.010.290822145
GTCTGAA488800.010.249167145
CCAGTCA259000.010.245262145
ACGTCTG500000.010.106586145
TCCAGTC275600.010.04907145
TCTCGTA36350.09.972575145
CACACGT620800.09.961828145
TGAACTC406900.09.960149145
TCTGAAC448400.09.943773145
CAGTCAC255650.09.897405145
Produced by FastQC (version 0.11.5)