FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7375824
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG19800.030.390953145
TTGGAGG122450.011.8558561
AGTGTAG199350.011.201234145
CGGTGGT91050.010.192047145
GTAGATC218600.010.181684145
GTGTAGG459050.09.965538145
GAGTGTA210550.09.916733145
GCCGTAT31450.09.912408145
GATCTCG126650.09.845883145
TCGTGTA502100.09.8041725145
TTGGGGG85300.09.7861191
CGTGTAG475200.09.733647145
TCTCGGT104300.09.731395145
GTGTAGA196650.09.6960125145
GTAGGGA407600.09.693792145
TAGGGAA385700.09.661507145
TGTAGGG437900.09.503166145
CGTCGTG586850.09.500154145
TGGTCGC56950.09.420401145
GTCGTGT534650.09.383566145
Produced by FastQC (version 0.11.5)