FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391732
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA48800.014.113822145
TCGTATG41250.013.0061455145
CTCGTAT44800.012.299187145
CGTATGC35100.012.186689145
ACGAGCT151100.012.139391145
CACGAGC163500.011.972555145
TATGCCG29150.011.689613145
ATCTCGT52850.011.523254145
TCACGAG181350.011.433766145
GTCACGA184800.011.18108145
AGTCACG209600.010.688286145
ATGCCGT40900.010.458503145
CGAGCTG162150.010.328466145
CGTCTGA489650.09.846383145
CAGTCAC293400.09.464105145
GTATGCC47250.09.052969145
CCAGTCA296200.08.983009145
TCCAGTC319300.08.946191145
CACGTCT567600.08.864574145
CTCCAGT357050.08.751633145
Produced by FastQC (version 0.11.5)