FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5261936
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAATCTCGTAT121360.23063754481240364TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTT220600.013.736554145
CTCGTAT87300.013.369608145
CGCTTAA179000.012.79797145
ATCTCGT101350.012.6606655145
ACGCTTA196250.012.374913145
TCTCGTA90800.012.215539145
CAACGCT239200.012.153189145
TATGCCG61150.011.973799145
TCGTATG80800.011.843215145
ACAACGC260600.011.572468145
CACAACG285500.011.045622145
AATCTCG122350.010.665361145
CGTATGC72950.010.335109145
GTCACAA362000.010.233386145
AGTCACA415000.010.009527145
GCTTAAT181850.09.92643145
TCACAAC333750.09.904912145
CAGTCAC425950.09.837308145
CTTAATC173750.09.805057145
CCAGTCA457700.09.645914145
Produced by FastQC (version 0.11.5)