FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8094226
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG76100.013.908963145
ATCTCGT99950.013.708993145
CGTATGC67550.012.557033145
TATGCCG56350.012.351049145
CTCGTAT85050.011.678116145
CGCCACA242600.011.594913145
TCTCGTA91500.011.568145
ACGCCAC267000.011.26843145
CCACATA228150.010.327363145
AGTCACG338300.09.857854145
CCAGTCA462800.09.587042145
TAATCTC153450.09.401837145
CACATAA205850.09.297788145
TCCAGTC490000.09.291592145
CAGTCAC451450.09.08936145
GCCACAT262950.09.043306145
ATAATCT174550.08.971404145
TCACGCC373200.08.877713145
GTCACGC359600.08.729608145
ATGCCGT70900.08.69159145
Produced by FastQC (version 0.11.5)