FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5425351
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTAT199810.3682895355526306TruSeq Adapter, Index 1 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAAA18950.024.101784145
CGCAATA8107.2759576E-1215.215342145
CGCAATC393500.09.432853145
CTCGTAT274550.09.16275145
CGTATGC243950.09.123371145
GTATGCC240500.08.98295145
TATGCCG211950.08.961603145
TCTCGTA283200.08.934083145
TCACGCA506150.08.7944145145
GTTCGCC6600.0085757358.7874718
ATCTCGT301800.08.743795145
CACGCAA470200.08.726732145
TCGTATG259400.08.69177145
ATGCCGT213750.08.614804145
GCCGTCT187150.08.599654145
ACGCAAT414900.08.282334145
CTTCACG582150.08.268962145
AATCTCG362750.08.193968145
ACTTCAC630550.08.140134145
TTCACGC547000.08.084634145
Produced by FastQC (version 0.11.5)