FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7074402
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCCG195100.013.526005145
CCGTCTA128350.013.386834145
ATCTCGT63650.013.326401145
CGTCTAA121050.012.816646145
TCGTATG50250.012.551874145
ACTCCGT183600.011.806575145
GTCTAAT111450.011.383698145
TCTCGTA59800.010.668586145
TCACTCC271050.010.217375145
TCTAATC104300.09.939788145
TCCGTCT180750.09.866933145
TCCAGTC371100.09.807045145
AGTCACT298850.09.800619145
CTCCGTC200700.09.60859145
CGTCTGA593050.09.486277145
CGTATGC56650.09.470158145
AACTCCA460600.09.459669145
CAGTCAC349500.09.293006145
CTCCAGT411950.09.221725145
CTAATCT102900.09.159113145
Produced by FastQC (version 0.11.5)