FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005688739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65909947
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG383350.010.798534145
CGTCTGA3538700.010.724996145
CTCGTAT265150.010.690777145
CGTATGC229650.010.29142145
ACGTCTG3858800.010.235498145
CACGTCT4177450.010.077776145
ACACGTC4377050.09.863349145
CCAGTCA2125000.09.8050995145
TCGTATG252550.09.731426145
GTCACAT1630300.09.71646145
CACACGT4820300.09.55797145
TCCAGTC2294950.09.502301145
CTGAACT3536500.09.481177145
TCTGAAC3629250.09.452616145
GAACTCC3182600.09.45117145
GCACACG5084600.09.423303145
ATCTCGT371800.09.379071145
TCTCGTA308150.09.316593145
ATGCCTA1072100.09.311567145
GTCTGAA3883400.09.229762145
Produced by FastQC (version 0.11.5)