FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7545989
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG26700.035.300415145
AGAGCGG57850.010.0261545145
AGAGCGC49850.08.435494145
CGTATCA206900.08.26988145
TGTAGAT689500.08.044052145
GTGTAGA683300.07.8305573145
GTCGCCG280700.07.8003173145
GTGGTCG327800.07.7854085145
TCGGTGG416050.07.6152406145
TAGATCT570150.07.3881335145
TCGCCGG22751.0859367E-97.3298345145
GATCTCG528500.07.2570214145
CGCCGTA235250.07.242458145
GAGTGTA737800.07.242301145
AGAGTGT805400.07.174546145
GCCGTAT225650.07.165017145
AGTGTAG706300.07.0828433145
GGGAAAG1069000.07.067043145
AAGAGTG889500.07.034165145
TCGCGTA8050.07.024825140-144
Produced by FastQC (version 0.11.5)