FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7239005
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG19750.036.34441145
GTCGCCG142800.013.810525145
TGGTCGC161750.013.626955145
GCCGTAT89200.013.167996145
CGCCGTA96400.013.0870495145
CGTATCA77750.013.05561145
CCGTATC86200.013.037489145
GTGGTCG181950.012.791531145
CGGTGGT230700.012.539922145
TCGCCGT123950.012.401062145
AGAGCGG52600.011.992315145
GGTCGCC166050.011.527485145
CTCGGTG307600.010.842789145
TCGGTGG273750.010.488433145
TAGATCT420100.010.407207145
TCTCGGT296950.010.401495145
GATCTCG359750.010.278717145
AGTGTAG556950.010.128215145
GGTGGTC242100.010.122584145
GTGTAGA530050.010.067705145
Produced by FastQC (version 0.11.5)