FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955888
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT182600.014.093775145
TATGCCG120650.013.939924145
ATCTCGT215050.012.910976145
CGTATGC151000.012.7704145
GTATGCC155650.012.575186145
TCTCGTA195650.012.338551145
TCGTATG166500.012.321737145
ATGCCGT125650.012.115926145
TGGTCAA436000.011.406106145
AATCTCG279300.011.264672145
GTCAATC326000.010.962992145
GCCGTCT110150.010.925048145
GTGGTCA473350.010.8124145
AGTGGTC501550.010.421273145
TCAATCT329450.010.320082145
GGTCAAT361000.010.020586145
TGCCGTC132450.09.742441145
TCACAGT730700.09.732617145
CAATCTC323550.09.56723145
CAGTGGT604250.09.549842145
Produced by FastQC (version 0.11.5)