FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7955888
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG24750.031.931225145
GCCGTAT133400.014.076938145
GTCGCCG189900.013.554006145
CGTATCA113000.013.345941145
GTGGTCG251100.013.282366145
CCGTATC125500.012.998795145
TGGTCGC222600.012.963497145
CGCCGTA141950.012.871509145
GGTCGCC215400.011.882102145
TCTCGGT396250.011.783678145
CGGTGGT312650.011.455995145
TCGCCGT168450.011.1479145
TCGGTGG351550.010.992702145
CTCGGTG387400.010.611768145
GATCTCG487800.010.538256145
GGTGGTC319700.010.454965145
AGATCTC551000.010.369049145
ATCTCGG508400.010.096992145
TAGATCT578950.010.0187435145
TGTAGAT727800.09.693153145
Produced by FastQC (version 0.11.5)