FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5437534
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT51450.014.232301145
TATGCCG32200.013.734506145
TCGTATG46000.013.554381145
ACGAATC160600.013.272137145
CGTATGC40600.013.035747145
CGAATCT153650.012.645655145
GTCACGA212750.012.574648145
ATCTCGT60200.012.284083145
CACGAAT177800.012.232877145
TCACGAA209900.012.227282145
TCTCGTA55550.012.137743145
AGTCACG238400.011.617059145
ATGCCGT40950.010.799782145
CAGTCAC313650.09.685183145
GCCGTCT37950.09.552066145
AATCTGA180500.09.479248145
TCCAGTC344500.09.322955145
TGAATCT127100.09.297819145
GTATGCC51650.08.843197145
AACTCCA419800.08.755977145
Produced by FastQC (version 0.11.5)