FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5437534
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG11750.025.297972145
CGCCGTA37800.014.001377145
CGTATCA32950.012.76181145
GCCGTAT35350.012.510658145
CGGTGGT98650.012.346729145
CCGTATC36700.011.852908145
TGGTCGC64900.011.617918145
TCTCGGT127700.011.46833145
GTCGCCG63250.011.462495145
GTGGTCG74200.011.334277145
TCGCCGT53400.011.133002145
TAGATCT193700.011.079031145
GTGTAGA244400.011.064896145
AGTGTAG255450.010.8133135145
GAGTGTA273200.010.721126145
TCGGTGG117700.010.717969145
GATCTCG158100.010.638878145
TGTAGAT252500.010.537665145
AGATCTC183950.010.168565145
GTAGATC252550.010.047555145
Produced by FastQC (version 0.11.5)