FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6604893
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15150.031.58245145
CGCCGTA30650.017.030075145
TCTCGGT125300.014.695933145
TGGTCGC61650.013.288008145
GCCGTAT30950.013.117224145
GTGGTCG70250.012.383673145
GTCGCCG56700.012.018751145
GGTCGCC69200.011.942996145
CGTATCA28100.011.3517145
GAGTGTA284550.011.235556145
CTCGGTG140850.011.220551145
TAGATCT201900.011.202961145
TGTAGAT271200.011.200537145
AGTGTAG269000.011.13044145
AGATCTC197950.010.877159145
CGGTGGT100800.010.788107145
GTGTAGA258350.010.691313145
TCGCCGT50750.010.570857145
GATCTCG169550.010.561211145
GTAGATC278250.010.42172145
Produced by FastQC (version 0.11.5)