FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8073157
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG24700.034.048428145
GCCGTAT42400.014.363142145
CGCCGTA43050.014.314685145
GTGGTCG96100.014.107636145
TCGGTGG160650.012.094561145
TGGTCGC81050.012.075824145
GGTCGCC89450.011.914422145
CGGTGGT131550.011.738829145
TCTCGGT169400.011.469842145
GAGTGTA381500.011.421311145
GATCTCG212700.011.4186125145
GTGTAGA341550.011.271358145
AGTGTAG353900.011.226283145
AGATCTC258650.011.183981145
GTCGCCG68900.011.048571145
TCGCCGT61000.010.934376145
CTCGGTG187750.010.889431145
TAGATCT266300.010.781024145
GTAGATC373400.010.775928145
ATCTCGG246750.010.342405145
Produced by FastQC (version 0.11.5)