FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8731993
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG480350.011.077985145
CGTCTGA437900.010.711538145
TCTGAAC441350.010.709939145
ACACGTC544150.010.565185145
CACGTCT527150.010.177006145
CACACGT598000.010.013858145
GTCTGAA476100.09.71505145
TCGTATG39050.09.653945145
TCCAGTC288100.09.56231145
GCACACG653150.09.257114145
CCAGTCA266500.09.221997145
CTGAACT429900.09.207626145
AGTCACT241900.09.170818145
AACTCCA362200.09.107213145
AGCACAC744700.08.742135145
CTCCAGT332350.08.72543145
GAACTCC388650.08.673948145
TCTCGTA43900.08.587393145
CTCGTAT40650.08.560581145
TGAACTC411550.08.367458145
Produced by FastQC (version 0.11.5)