FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8511045
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC14650.031.6686656
GCGTCGG20500.024.050924145
CCGATCT21100.021.987837
TTCCGAT22450.021.6347065
CTTCCGA34250.014.3926534
CGATCTA10806.366463E-1112.0818568
CGCCGTA52600.011.716825145
AGTGTAG298950.010.5503645145
GATCTCG187550.010.438148145
CGTATCA47050.010.325044145
GAGTGTA319450.09.964107145
CGGTGGT132550.09.900921145
GTCGCCG81800.09.838897145
GTGGTCG102750.09.526397145
TCGCCGT71550.09.525657145
TAGATCT233250.09.512101145
TCTTCCG54400.09.4634373
GTGTAGA291400.09.455201145
GCCGTAT51400.09.451232145
GTGTAGG641200.09.396892145
Produced by FastQC (version 0.11.5)