FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7405650
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA51200.014.160132145
TCGTATG39450.013.967022145
CTAAGGT136750.013.731238145
TATGCCG27500.013.709067145
ACCTAAG177000.013.435005145
TAAGGTC123550.013.320496145
CTCGTAT44600.012.67935145
TCACCTA224450.012.532837145
CCTAAGG161350.012.40159145
CACCTAA195050.012.117385145
ATCTCGT54350.012.005499145
CGTATGC34100.010.843091145
AGTCACC288000.010.673592145
CATCTCG75450.010.665985145
CTCCAGT420150.010.18087145
CAGTCAC341300.010.111321145
GTCACCT273450.010.101464145
TGAACTC546950.09.967988145
GAACTCC516200.09.957849145
AACTCCA473700.09.8717375145
Produced by FastQC (version 0.11.5)