FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7129266
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG13450.023.716997145
CCGTATC36250.011.799764145
CGCCGTA34450.011.364068145
GCCGTAT35800.011.340554145
TCGCCGT52600.010.199415145
TGGTCGC60600.010.049303145
AGTGTAG215250.09.969604145
GTGGTCG71700.09.909146145
GTGTAGA214650.09.86237145
GAGTGTA228450.09.837842145
GATCTCG138900.09.708229145
GTAGATC219550.09.609236145
TCTCGGT117250.09.522198145
CGTATCA34400.09.27307145
AGAGTGT264350.09.10517145
GGTCGCC74200.08.989038145
TAGATCT175500.08.964208145
TCGGTGG121100.08.800402145
CGTCGTG543800.08.75902145
TCGTGTA475450.08.676344145
Produced by FastQC (version 0.11.5)