FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7369525
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC39050.016.337622145
TATGCCG31400.013.391386145
TCTCGTA54750.011.917511145
ATCTCGT59800.011.517272145
TCGTATG44650.011.203528145
CTCGTAT48200.010.980017145
GTCACAG281750.09.932317145
CGTCTGA586550.09.826284145
AGAGTCA199400.09.634915145
CCAGTCA340050.09.487339145
TCCAGTC364600.09.484817145
TGAACTC518350.09.230985145
CTCCAGT413600.09.220032145
GAGTCAA163700.09.167457145
AGTCACA304650.09.090533145
AACTCCA453400.09.018302145
GAACTCC493750.08.986106145
ACGTCTG632200.08.979135145
CAGTCAC336100.08.973296145
CACGTCT682650.08.889034145
Produced by FastQC (version 0.11.5)