FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6711204
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG149050.013.425236145
TCGTATG31700.012.579062145
TATGCCG23100.012.554309145
CACGACT109650.012.430655145
CTCGTAT35850.012.336317145
CGACTAG91750.012.327168145
ACGACTA97450.012.052524145
GTCACGA126550.011.51539145
ACTAGTA87500.010.937314145
TCACGAC116650.010.814571145
CGTCTGA350750.010.211137145
GACTAGT89950.010.075199145
ACGTCTG379150.09.771352145
ACACGTC427850.09.404729145
CTAGTAA86700.09.282152145
AGTAATC86000.09.02049145
CACGTCT417400.08.893288145
ATCTCGT41200.08.798681145
CCAGTCA227350.08.673986145
GCACACG498250.08.628836145
Produced by FastQC (version 0.11.5)