FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6290776
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52100.015.863522145
CTCGTAT57500.014.37373145
ATCTCGT69550.013.655468145
TCTCGTA63850.013.171328145
CGTATGC44750.012.798718145
TATGCCG36300.012.582483145
CGTCTGT201950.012.56483145
GTCACGT269050.012.071952145
AGTCACG290100.011.695817145
GTCAATC119850.011.614369145
TCAATCT129500.011.420704145
TCACGTC262650.010.461507145
CTGTCAA185950.010.409924145
GTCTGTC213000.010.347286145
GTATGCC55500.010.189058145
ATGCCGT49000.010.061096145
TGTCAAT159700.010.032745145
CCAGTCA391300.09.597373145
TCCAGTC416550.09.172251145
ACGTCTG917500.09.158194145
Produced by FastQC (version 0.11.5)