FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6290776
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG12600.024.741573145
GCCGTAT39250.014.222647145
CGCCGTA40250.014.049411145
CGTATCA34650.012.972325145
TCGCCGT60300.012.864587145
TGGTCGC76400.012.81061145
CGGTGGT118950.012.616396145
GTCGCCG69800.012.567802145
TCTCGGT153850.012.440442145
GTGGTCG88150.011.596479145
AGATCTC227300.011.57808145
GTGTAGA291650.011.1861315145
CTCGGTG165500.011.170473145
AGTGTAG305050.011.027481145
GATCTCG196650.010.986305145
CCGTATC40500.010.740527145
TAGATCT226650.010.555714145
TCGGTGG144000.010.472014145
GAGTGTA325000.010.4621145
GGTCGCC86050.010.278703145
Produced by FastQC (version 0.11.5)