FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8085596
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG43250.011.39846145
CTCGTAT47900.010.594634145
ATCTCGT54750.010.593252145
TATGCCG30900.010.557898145
AGTCACC273550.010.441981145
GAACTCC470950.010.3447145
GAGCATC118450.010.160064145
CATCTCG76750.09.823774145
TCTCGTA50450.09.771724145
TCCAGTC346550.09.706787145
TCTGAAC553950.09.658489145
CACGTCT661300.09.658303145
TGAACTC499800.09.617023145
CGTATGC37000.09.60103145
CACACGT744700.09.598845145
CGTCTGA563550.09.416772145
ACGTCTG606400.09.396948145
GTCTGAA593850.09.351375145
ACACGTC684900.09.3255005145
CTGAACT536400.09.217625145
Produced by FastQC (version 0.11.5)