FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7325735
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG21150.033.24985145
CGCCGTA27750.013.324027145
GATCTCG142550.012.307689145
AGTGTAG231400.012.250159145
GTGTAGA225050.011.4683075145
TGGTCGC55650.011.073428145
TAGATCT175150.010.8447485145
GTGGTCG62200.010.839791145
TCTCGGT119800.010.529816145
GAGTGTA254650.010.220664145
GTAGATC241950.010.2177925145
AGAGCGG45250.010.093698145
AAGAGTG355550.09.991341145
CGGACGA9502.4417022E-69.920832145
TCGGTGG116950.09.670585145
GTAGGGA479450.09.556571145
CGTGTAG551950.09.5097065145
TGTAGAT238250.09.433158145
AGAGTGT289900.09.428041145
CGGTGGT90900.09.411232145
Produced by FastQC (version 0.11.5)