FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8026317
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT56650.013.820692145
ATCTCGT69250.012.3528805145
TCTCGTA61350.012.171066145
TCGTATG50650.011.164034145
GTCACCT296650.010.972559145
AGTCACC314400.010.560609145
CGTCTGA672900.010.180925145
CTCAATG204900.010.118824145
CAGTCAC374950.010.053932145
TCCAGTC412100.010.044758145
CACCTCA287300.09.941835145
CGTATGC45150.09.794405145
ACGTCTG724750.09.5625925145
CCTCAAT212400.09.488472145
TCTGAAC648650.09.4774475145
GAACTCC552750.09.469225145
TGAACTC585600.09.457977145
ACCTCAA266250.09.257534145
TCAATGA219000.09.2025175145
AACTCCA512150.09.059177145
Produced by FastQC (version 0.11.5)