FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8026317
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG23400.028.815823145
TGGTCGC74150.012.222577145
TCGCGTA4804.3837645E-1012.084055145
GTGTAGA286700.011.531905145
TCTCGGT143950.011.483838145
GAGTGTA306950.011.4797535145
GATCTCG175700.011.471942145
AGAGCGG55050.011.45845145
CGCCGTA38400.011.328801145
GTAGATC302300.011.224619145
GTCGCCG68850.011.057304145
CGGTGGT112000.010.746177145
GTGGTCG82950.010.576279145
AGTGTAG294100.010.502156145
TAGATCT219350.010.478172145
CTCGGTG164300.010.105594145
GTGTAGG663000.010.039061145
GTAGGGA587000.09.943098145
AAGAGTG415250.09.812747145
TAGGGAA551800.09.736445145
Produced by FastQC (version 0.11.5)