FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7201330
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA326700.08.3215885145
GTCACGA325550.08.239637145
TATGCCG110050.08.23463145
CACGAAC295650.08.165645145
CTCGTAT137600.08.113839145
ACGAACA288050.08.02873145
CGTATGC123800.07.8470836145
AGTCACG351200.07.844282145
ATCTCGT147400.07.7219377145
CCAGTCA445500.07.713558145
TCCAGTC478050.07.7039685145
CGAACAG261550.07.567145145
TCTCGTA138050.07.404689145
ACACGTC779450.07.319992145
GTCTGAA684950.07.2714663145
CAGTCAC438650.07.2555523145
CACGTCT762250.07.1903243145
AACTCCA570250.07.1194563145
CGTCTGA670750.07.0795355145
ACGTCTG709200.06.98194145
Produced by FastQC (version 0.11.5)