FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7849284
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG17550.022.720116145
CCGTATC60450.014.0318365145
CGCCGTA61850.013.362575145
GCCGTAT59150.011.643777145
GTGGTCG118750.011.538602145
CGTATCA52450.011.472488145
GGTCGCC119000.011.392516145
GATCTCG243400.010.692983145
CGGTGGT162700.010.605091145
TGGTCGC105750.010.557603145
GTGTAGA360650.010.5334425145
TCTCGGT198150.010.281045145
TGTAGAT370000.010.247666145
TCGCCGT92300.010.210961145
AGATCTC282000.010.129129145
AGTGTAG373850.010.045172145
TAGATCT280900.09.9881315145
GTAGATC364350.09.809641145
CTCGGTG219550.09.642161145
TCGGTGG194800.09.639085145
Produced by FastQC (version 0.11.5)