FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6827589
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG13350.036.383076145
GCCGTAT30200.014.402908145
GTGGTCG66100.012.941554145
CGGTGGT87150.012.89348145
CGCCGTA28950.012.520662145
GTAGATC245300.011.644063145
CGTATCA28850.011.558937145
GAGTGTA259750.011.41494145
GATCTCG150150.011.394428145
TGGTCGC55400.011.384537145
AGATCTC178300.011.384462145
GTGTAGA235300.011.18383145
CCGTATC30600.011.134798145
GGTCGCC70300.010.82779145
TAGATCT184150.010.825971145
TCTCGGT119200.010.460635145
TCGCCGT49650.010.220794145
AGTGTAG239900.010.183699145
GTAGGGA489100.09.886306145
AGAGTGT297250.09.852929145
Produced by FastQC (version 0.11.5)