FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6273139
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG13200.025.264175145
CGCCGTA31300.013.202364145
TGGTCGC54700.011.398097145
GAGTGTA205600.011.318872145
CGTGTAG470050.011.228158145
GTGTAGA189800.010.886039145
AGAGTGT242500.010.732574145
AGTGTAG193350.010.423698145
TGTAGGG427650.010.391858145
GTAGATC206750.010.2039585145
TAGATCT146450.010.098619145
TCGTGTA498400.09.876725145
TAGGGAA376100.09.830782145
TGTAGAT205050.09.722862145
TCTCGGT98850.09.60762145
GTGGTCG62050.09.580613145
GTAGGGA398800.09.416637145
CGTCGTG578400.09.413105145
GTCGTGT531950.09.403715145
AAAGAGT292250.09.203238145
Produced by FastQC (version 0.11.5)