FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5427308
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG9900.027.82666145
CGCCGTA15750.016.11017145
AGTGTAG139800.012.912336145
TCTCGGT68050.011.931705145
GATCTCG87150.011.645906145
GTGTAGA135450.011.453743145
GTAGGGA310100.011.361799145
TAGGGAA290300.011.1628145
TGTAGGG333750.011.143169145
CGTATCA17000.011.087588145
TAGATCT109650.011.041307145
GAGTGTA150850.011.005323145
CGTGTAG365600.010.509507145
TGGTCGC32650.010.435838145
GCCGTAT16150.010.324473145
GTGTAGG351100.010.09696145
GTAGATC157500.010.080363145
TCGTGTA391050.010.048145
AGGGAAA314400.09.8229145
AGAGTGT178350.09.796176145
Produced by FastQC (version 0.11.5)