FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7041392
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG15300.028.43095145
GCCGTAT33300.015.675443145
TGGTCGC60550.013.889142145
CGCCGTA32350.011.653613145
CGGTGGT97000.011.58488145
TCTCGGT124150.011.4456625145
GAGTGTA263700.011.354591145
AGTGTAG245950.010.788618145
TCGCCGT51150.010.7720785145
GTGTAGA240450.010.673579145
GATCTCG156100.010.6356535145
GTGGTCG68900.010.627564145
AGAGTGT308750.010.284868145
GTGTAGG587700.010.263588145
GTAGATC262250.010.200993145
CCGTATC33700.010.111126145
TGTAGGG553950.010.077476145
AGATCTC188600.010.07143145
GTAGGGA516900.010.070463145
TCGGTGG119250.09.909706145
Produced by FastQC (version 0.11.5)