FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7495747
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG11400.039.430202145
GTCGCCG68300.010.721187145
CGCCGTA52450.010.643573145
TGGTCGC84150.09.563372145
GCAACAT99100.09.4495051
CGTGTAG500350.09.404008145
GTGTAGA246100.08.985255145
CGGTGGT117350.08.834768145
AGTGTAG250150.08.752832145
ACATTGT79600.08.7435694
TCGGTGG127400.08.706913145
GTAGGGA441200.08.67642145
CAACATT108100.08.585072
TCGTGTA526350.08.553801145
GTGTAGG487050.08.529493145
TAGGGAA426100.08.524489145
TCTCGGT137300.08.448733145
GAGTGTA258050.08.42868145
GATCTCG167900.08.247547145
TCGCCGT64350.08.224632145
Produced by FastQC (version 0.11.5)