FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6804397
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33600.014.672743145
TCGTATG49500.014.500123145
CTCGTAT54500.013.568922145
ATCTCGT63900.012.820922145
ACGATAG161500.012.43509145
CACGATA177300.012.431013145
TCACGAT198900.012.247464145
CGTATGC42100.012.226944145
CGATAGA149600.012.018819145
GATAGAC151800.011.653589145
ATAGACA156200.011.603811145
AGTCACG239000.011.436289145
GTCACGA212100.011.075058145
GACAATC114700.010.303052145
TCTCGTA57950.010.1338215145
TAGACAA151250.09.347186145
ATGCCGT45100.09.323804145
CAGTCAC330100.08.829218145
CGTCTGA540800.08.7408285145
AACTCCA429800.08.653517145
Produced by FastQC (version 0.11.5)