FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7275008
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA91900.015.541388145
CCGTCTA101250.013.891393145
ATCTCGT47000.013.883014145
TCTCGTA41200.013.725762145
CACTCCG161550.013.642869145
CGTATGC27800.013.561184145
TATGCCG22000.013.511396145
TCTAATC89850.013.475269145
ACTCCGT155900.013.160712145
TCGTATG32700.013.081073145
CTCCGTC157950.011.934185145
TCACTCC228450.011.615262145
TCCGTCT141250.011.240736145
AGTCACT276650.011.190159145
CTCGTAT37100.010.943422145
CCAGTCA327950.010.876684145
CTCCAGT400950.010.776932145
CAGTCAC315350.010.690529145
GTCACTC245200.010.673966145
CTAATCT88300.010.673865145
Produced by FastQC (version 0.11.5)