FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9300047
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG23750.027.475212145
CGCCGTA57650.014.08578145
TGGTCGC103450.013.246267145
GATCTCG247850.012.842152145
GTGGTCG118600.012.654583145
CGTATCA52850.012.072574145
TCTCGGT203500.011.828669145
GCCGTAT55800.011.824136145
GAGTGTA442600.011.614407145
CGGTGGT156650.011.4784565145
GTCGCCG97500.011.22883145
AGTGTAG414400.011.12755145
TCGCCGT84450.010.903508145
TAGATCT317650.010.864763145
TCGGTGG191450.010.641752145
GTGTAGA399350.010.620975145
GGTCGCC113950.010.562239145
AGATCTC305550.010.298397145
CTCGGTG218550.010.184736145
GTAGATC433750.09.962515145
Produced by FastQC (version 0.11.5)