FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7463755
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC13400.045.9858176
TAGAGGG51500.037.869013
CCGATCT17250.036.143797
ATAGAGG52700.036.0437162
GATAGAG62900.030.1198521
TTCCGAT21800.028.5990585
GCGTCGG19550.028.184013145
CTTCCGA32800.019.229194
AGAGGGG125450.014.6206094
CGATCTA11900.013.4029418
TCTTCCG50150.013.15556053
TGGTCGC78150.011.7817745145
GTGGTCG90300.011.15997145
CGTATCA41000.011.140191145
GCCGTAT45550.010.982386145
GAGGGGG158250.010.8571155
GATCTCG178550.010.719637145
TAGATCT218550.010.449505145
GTGTAGA273500.010.444193145
TCTCGGT150150.09.994958145
Produced by FastQC (version 0.11.5)