FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7771436
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG20600.026.74904145
CGCCGTA45500.014.500794145
GCCGTAT44900.013.5642185145
CGTATCA38650.012.943789145
GGTCGCC98400.012.894507145
CCGTATC44600.012.680068145
TGGTCGC86150.012.455703145
GTGGTCG99150.012.211966145
TAGATCT256050.011.524748145
TCTCGGT170300.011.324755145
GATCTCG205100.011.206123145
GTCGCCG79550.011.119402145
CGGTGGT130800.010.975372145
GTGTAGA328000.010.897701145
GAGTGTA359450.010.791384145
TCGCCGT68650.010.667008145
TCGGTGG164100.010.515507145
AGTGTAG339900.010.388183145
CTCGGTG189000.010.35771145
AGAGTGT415150.010.234212145
Produced by FastQC (version 0.11.5)