FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7023366
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTAT99910.14225372848289553TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG63100.012.178746145
ATCTCGT81300.011.860067145
CTCGTAT68100.011.178106145
CACAATG287450.011.021613145
TCTCGTA73600.010.638294145
CGTATGC55400.010.599889145
CAGTCAC402000.010.550082145
GTCACAA317400.010.347069145
CAATGTT251400.010.323901145
GTTGCAT152250.010.285431145
TCACAAT313950.010.252942145
CCAGTCA419500.010.0926895145
TTGCATC162200.010.012054145
CATCTCG100400.09.964865145
TATGCCG46650.09.9461355145
ACAATGT256700.09.91305145
AATGTTG252250.09.858004145
TGTTGCA202000.09.833887145
AGTCACA370750.09.757654145
TCCAGTC453950.09.646172145
Produced by FastQC (version 0.11.5)