FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7529557
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC42850.012.858485145
TCGTATG50050.012.746932145
ATCTCGT62750.012.477775145
CACCTTA159350.011.601527145
TATGCCG33150.011.590959145
ACCTTAC138300.011.218083145
CTCGTAT53900.011.163912145
CCTTACA141600.011.059043145
CATCTCG86100.011.0305145
AGTCACC290100.010.471127145
CCAGTCA351350.09.966307145
GTCACCA300750.09.714635145
TCCAGTC377650.09.368227145
TCTCGTA59350.09.283675145
CAGTCAC344850.09.271189145
CGTCTGA601600.09.194832145
CTCCAGT427600.09.138568145
GTATGCC54900.09.111793145
TGAACTC537350.09.052997145
CTGAACT565850.08.930144145
Produced by FastQC (version 0.11.5)