FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7488902
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT61100.014.475829145
CTAATCC137750.012.841716145
TATGCCG34000.012.79373145
ACCCTAA177250.011.738712145
CTCGTAT50650.011.737058145
CCTAATC148750.011.648386145
CACCCTA189950.011.4882145
CCCTAAT159750.011.436273145
CGTATGC40450.010.9329195145
TCGTATG45200.010.90675145
AGTCACC281700.09.959762145
CGTCTGA568000.09.828048145
TCTCGTA57400.09.472709145
CTCCAGT406600.09.23607145
TCCAGTC358400.09.163365145
CACGTCT661800.09.125215145
CTGAACT539050.09.105095145
CCAGTCA338000.09.0943985145
TAATCCA151600.08.990493145
GTCACCC285000.08.903239145
Produced by FastQC (version 0.11.5)