FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7116998
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG52900.015.760433145
CTCGTAT59600.013.988706145
CGAGTTA169600.013.849853145
AGTTAGC155800.013.075697145
ATCTCGT69200.012.99097145
CGTATGC45250.012.496891145
ACGAGTT194000.012.4442425145
TATGCCG37400.012.212228145
TCACGAG238400.012.012041145
GTTAGCA145700.011.991775145
CATCTCG93700.011.528496145
GTCACGA248450.011.526144145
TCTCGTA64350.011.491523145
AGTCACG280050.011.468169145
CACGAGT224150.011.255544145
GAGTTAG175150.011.051649145
TTAGCAT139600.010.386531145
ATGCCGT53750.010.385758145
TCCAGTC412500.010.000329145
TAGCATC126850.09.715931145
Produced by FastQC (version 0.11.5)