FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7112357
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC39550.015.397921145
CTCGTAT51500.014.21816145
CGACAAC133100.013.453898145
TCGTATG45300.013.443438145
TCTCGTA57250.012.536865145
ACCCGAC176400.012.288584145
CACCCGA196150.012.08617145
CCCGACA173700.011.937008145
CCGACAA158850.011.8206625145
ATCTCGT62250.011.646352145
ATGCCGT45150.011.240084145
TCACCCG216950.011.061081145
TATGCCG31000.010.757847145
AGTCACC291500.010.594983145
TCCAGTC375300.09.909872145
GTATGCC53850.09.558767145
CCAGTCA353450.09.496908145
CATCTCG84250.09.465685145
GTCACCC295250.09.453662145
CAGTCAC345800.09.350593145
Produced by FastQC (version 0.11.5)