FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005688973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005688973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7472858
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT54550.014.220499145
TCGTATG41700.013.560771145
TCTCGTA50600.012.321789145
CTCGTAT46050.011.807486145
GGCTAAC121950.011.770887145
CGTATGC35600.011.200529145
CAGGCTA170200.010.350766145
AGGCTAA145150.010.039332145
CATCTCG78900.09.739903145
TCCAGTC324550.09.650015145
GCTAACA123100.09.599648145
AGTCACA280850.09.3445835145
AACTCCA406650.09.110159145
TCTGAAC498500.09.089514145
ACGTCTG539150.09.04964145
CGTCTGA500850.08.974491145
TATGCCG28850.08.795247145
TGAACTC461400.08.783347145
GAACTCC436800.08.697101145
GTCACAG263850.08.68272145
Produced by FastQC (version 0.11.5)